A practitioner's case for one fragment screening method and library size
- A single-author perspective on two basic FBDD design choices: the screening method and the size of the fragment library.
- The author lays out the method and library size used in their own lab, with reasons for each.
- A worked example illustrates the benefits, offered as one viewpoint since practice varies widely.
💡 Points Based on the abstract
Library size means little without hit rate and ligandability data: a few hundred well-curated fragments that give clean, soluble, orthogonally confirmed binders can beat thousands run through one noisy readout. For anyone choosing a setup, the numbers to ask for are how many hits were crystallographically validated and their ligand efficiencies, since those decide whether a fragment can be grown.
What Method and Library Size Is Best for Fragment Screening? ↗